In traditional bottom-up experiments, proteins are initially extracted and digested by sequence-specific proteases. The resulting peptides are subsequently analyzed by liquid chromatography (LC) typically coupled to the mass spectrometer through an electrospray interface. The sample preparation prior to LC?MS analysis is a critical part of the overall workflow as it highly impacts sensitivity, robustness, and reproducibility of any bottom-up proteomics approach.
The Preciex? Cleanup tip columns use a proprietary mixed-bed Resin to clean peptide mixtures, specialize in high peptides recovery and efficiently remove detergents, polymers, salts, lipid and more for reliable LC-MS analyses. For detergents such as CHAPS, Igepal, SDC, SDS, Triton X-100 and the polymer PEG-10k the tip removed >99.5% of the contamination; for Tween-20, the removal efficiency was >85%, due to the high repeat numbers of polytheylene glycol.
Process of use: centrifugation, vacuum, positive pressure.
Formats: 10μL, 200μL, 350μL and 1mL.
For any other offline or online format, contact us.
*200μL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.
Features of Cleanup Stage-tip:
? Dual cleanup step removes hydrophilic and hydrophobic contaminants
? Achieve ready-to-measure peptides
? Reduce mass spectrometry downtime
? Suitable for manual and automated processing
Common product Specifications:
Sample | peptide |
Chromatographic bed | N/A |
Volume | 200μL |
Capacity | 10ug |
pH Range | 2-12 |
Temperature | 4-70°C |
Quantity | 96 Tips |
Detection Method | HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy |
Ordering Information:
Description | Format | Capacity | Part Number |
Preciex Cleanup | 10μL | 2μg | 2107021 |
Preciex Cleanup | 200μL | 10μg | 2107102 |
Preciex Cleanup | 200μL | 30μg | 2107303 |
Preciex Cleanup | 350μL | 100μg | 2107104 |
User Guide
http://www.ecmlsx.cn/public/upload/202212/20221205130519.pdf
Material Safety Data Sheets (MSDS)
